View source for 7odf


==Structure of the mini-RNA-guided endonuclease CRISPR-Cas_phi3==
<StructureSection load='7odf' size='340' side='right'caption='[[7odf]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7odf]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Phage_#D Phage #D] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ODF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ODF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7odf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7odf OCA], [https://pdbe.org/7odf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7odf RCSB], [https://www.ebi.ac.uk/pdbsum/7odf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7odf ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
CRISPR-Cas12j is a recently identified family of miniaturized RNA-guided endonucleases from phages. These ribonucleoproteins provide a compact scaffold gathering all key activities of a genome editing tool. We provide the first structural insight into the Cas12j family by determining the cryoEM structure of Cas12j3/R-loop complex after DNA cleavage. The structure reveals the machinery for PAM recognition, hybrid assembly and DNA cleavage. The crRNA-DNA hybrid is directed to the stop domain that splits the hybrid, guiding the T-strand towards the catalytic site. The conserved RuvC insertion is anchored in the stop domain and interacts along the phosphate backbone of the crRNA in the hybrid. The assembly of a hybrid longer than 12-nt activates catalysis through key functional residues in the RuvC insertion. Our findings suggest why Cas12j unleashes unspecific ssDNA degradation after activation. A site-directed mutagenesis analysis supports the DNA cutting mechanism, providing new avenues to redesign CRISPR-Cas12j nucleases for genome editing.

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3.,Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G Nat Commun. 2021 Jul 22;12(1):4476. doi: 10.1038/s41467-021-24707-3. PMID:34294706<ref>PMID:34294706</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7odf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Phage #D]]
[[Category: Synthetic construct]]
[[Category: Carabias del Rey A]]
[[Category: Erledsson S]]
[[Category: Fugilsang A]]
[[Category: Montoya G]]
[[Category: Pape T]]
[[Category: Sofos N]]
[[Category: Stella S]]
[[Category: Temperini P]]