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==Cryo-EM structure of the Sulfolobus acidocaldarius RNA polymerase at 2.88 A==
<StructureSection load='7ok0' size='340' side='right'caption='[[7ok0]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ok0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OK0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OK0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ok0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ok0 OCA], [https://pdbe.org/7ok0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ok0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ok0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ok0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPO1N_SULAC RPO1N_SULAC] DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.[HAMAP-Rule:MF_00863]<ref>PMID:34535646</ref> <ref>PMID:34535646</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RNA polymerase inhibition plays an important role in the regulation of transcription in response to environmental changes and in the virus-host relationship. Here we present the high-resolution structures of two such RNAP-inhibitor complexes that provide the structural bases underlying RNAP inhibition in archaea. The Acidianus two-tailed virus encodes the RIP factor that binds inside the DNA-binding channel of RNAP, inhibiting transcription by occlusion of binding sites for nucleic acid and the transcription initiation factor TFB. Infection with the Sulfolobus Turreted Icosahedral Virus induces the expression of the host factor TFS4, which binds in the RNAP funnel similarly to eukaryotic transcript cleavage factors. However, TFS4 allosterically induces a widening of the DNA-binding channel which disrupts trigger loop and bridge helix motifs. Importantly, the conformational changes induced by TFS4 are closely related to inactivated states of RNAP in other domains of life indicating a deep evolutionary conservation of allosteric RNAP inhibition.

Structural basis of RNA polymerase inhibition by viral and host factors.,Pilotto S, Fouqueau T, Lukoyanova N, Sheppard C, Lucas-Staat S, Diaz-Santin LM, Matelska D, Prangishvili D, Cheung ACM, Werner F Nat Commun. 2021 Sep 17;12(1):5523. doi: 10.1038/s41467-021-25666-5. PMID:34535646<ref>PMID:34535646</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ok0" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus acidocaldarius DSM 639]]
[[Category: Cheung ACM]]
[[Category: Diaz-Santin LM]]
[[Category: Fouqueau T]]
[[Category: Lucas-Staat S]]
[[Category: Lukoyanova N]]
[[Category: Matelska D]]
[[Category: Pilotto S]]
[[Category: Prangishvili D]]
[[Category: Sheppard C]]
[[Category: Werner F]]