View source for 7p1w


==Crystal structure of a R51 R53 double mutant of the DNA-binding protein RemA from Geobacillus thermodenitrificans==
<StructureSection load='7p1w' size='340' side='right'caption='[[7p1w]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7p1w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P1W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P1W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p1w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p1w OCA], [https://pdbe.org/7p1w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p1w RCSB], [https://www.ebi.ac.uk/pdbsum/7p1w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p1w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y1019_GEOTN Y1019_GEOTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain.

Structural and functional characterization of the bacterial biofilm activator RemA.,Hoffmann T, Mrusek D, Bedrunka P, Burchert F, Mais CN, Kearns DB, Altegoer F, Bremer E, Bange G Nat Commun. 2021 Sep 29;12(1):5707. doi: 10.1038/s41467-021-26005-4. PMID:34588455<ref>PMID:34588455</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7p1w" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus thermodenitrificans NG80-2]]
[[Category: Large Structures]]
[[Category: Altegoer F]]
[[Category: Bange G]]
[[Category: Mrusek D]]