View source for 7p3x


==Homology model of the full-length AP-3 complex in a compact open conformation==
<StructureSection load='7p3x' size='340' side='right'caption='[[7p3x]], [[Resolution|resolution]] 9.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7p3x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P3X FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p3x OCA], [https://pdbe.org/7p3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p3x RCSB], [https://www.ebi.ac.uk/pdbsum/7p3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p3x ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AP3D_YEAST AP3D_YEAST] Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.<ref>PMID:10559961</ref> <ref>PMID:9250663</ref> <ref>PMID:9335339</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vesicle formation at endomembranes requires the selective concentration of cargo by coat proteins. Conserved adapter protein complexes at the Golgi (AP-3), the endosome (AP-1), or the plasma membrane (AP-2) with their conserved core domain and flexible ear domains mediate this function. These complexes also rely on the small GTPase Arf1 and/or specific phosphoinositides for membrane binding. The structural details that influence these processes, however, are still poorly understood. Here we present cryo-EM structures of the full-length stable 300 kDa yeast AP-3 complex. The structures reveal that AP-3 adopts an open conformation in solution, comparable to the membrane-bound conformations of AP-1 or AP-2. This open conformation appears to be far more flexible than AP-1 or AP-2, resulting in compact, intermediate, and stretched subconformations. Mass spectrometrical analysis of the cross-linked AP-3 complex further indicates that the ear domains are flexibly attached to the surface of the complex. Using biochemical reconstitution assays, we also show that efficient AP-3 recruitment to the membrane depends primarily on cargo binding. Once bound to cargo, AP-3 clustered and immobilized cargo molecules, as revealed by single-molecule imaging on polymer-supported membranes. We conclude that its flexible open state may enable AP-3 to bind and collect cargo at the Golgi and could thus allow coordinated vesicle formation at the trans-Golgi upon Arf1 activation.

Flexible open conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi.,Schoppe J, Schubert E, Apelbaum A, Yavavli E, Birkholz O, Stephanowitz H, Han Y, Perz A, Hofnagel O, Liu F, Piehler J, Raunser S, Ungermann C J Biol Chem. 2021 Nov;297(5):101334. doi: 10.1016/j.jbc.2021.101334. Epub 2021 , Oct 22. PMID:34688652<ref>PMID:34688652</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7p3x" style="background-color:#fffaf0;"></div>

==See Also==
*[[Adaptin 3D structures|Adaptin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Raunser S]]
[[Category: Schubert E]]