View source for 7pkr


==Vault structure in primmed conformation==
<StructureSection load='7pkr' size='340' side='right'caption='[[7pkr]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PKR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pkr OCA], [https://pdbe.org/7pkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pkr RCSB], [https://www.ebi.ac.uk/pdbsum/7pkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pkr ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Vaults are ubiquitous ribonucleoprotein particles involved in a diversity of cellular processes, with promising applications as nanodevices for delivery of multiple cargos. The vault shell is assembled by the symmetrical association of multiple copies of the major vault protein that, initially, generates half vaults. The pairwise, anti-parallel association of two half vaults produces whole vaults. Here, using a combination of vault recombinant reconstitution and structural techniques, we characterized the molecular determinants for the vault opening process. This process commences with a relaxation of the vault waist, causing the expansion of the inner cavity. Then, local disengagement of amino-terminal domains at the vault midsection seeds a conformational change that leads to the aperture, facilitating access to the inner cavity where cargo is hosted. These results inform a hitherto uncharacterized step of the vault cycle and will aid current engineering efforts leveraging vault for tailored cargo delivery.

Symmetry disruption commits vault particles to disassembly.,Guerra P, Gonzalez-Alamos M, Llauro A, Casanas A, Querol-Audi J, de Pablo PJ, Verdaguer N Sci Adv. 2022 Feb 11;8(6):eabj7795. doi: 10.1126/sciadv.abj7795. Epub 2022 Feb 9. PMID:35138889<ref>PMID:35138889</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7pkr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Casanas A]]
[[Category: Gonzalez-Alamos M]]
[[Category: Guerra P]]
[[Category: Llauro A]]
[[Category: Querol-Audi J]]
[[Category: Verdaguer N]]
[[Category: De Pablo P]]