View source for 7pkw


==Crystal structure of VIRB8-like OrfG central and C-terminal domains of Streptococcus thermophilus ICESt3 (Gram positive conjugative type IV secretion system).==
<StructureSection load='7pkw' size='340' side='right'caption='[[7pkw]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7pkw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PKW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.835&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pkw OCA], [https://pdbe.org/7pkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pkw RCSB], [https://www.ebi.ac.uk/pdbsum/7pkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pkw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q70CA4_STRTR Q70CA4_STRTR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial type IV secretion systems (T4SSs) are widespread nanomachines specialized in the transport across the cell envelope of various types of molecules including mobile genetic elements during conjugation. Despite their prevalence in Gram-positive bacteria, including relevant pathogens, their assembly and functioning remain unknown. This study addresses these gaps by investigating VirB8 proteins, known to be central components of conjugative T4SSs in Gram-positive bacteria. However, the functional packing and precise role of VirB8 in T4SSs biology remain undefined. Our findings elucidate the nature of VirB8 proteins as cell wall components, where they multimerize and exhibit a conserved assembly pattern, distinct from VirB8 in Gram-negative bacteria. We also demonstrate that VirB8 proteins interact with other T4SS subunits and DNA, indicating their pivotal role in the building of the DNA translocation channel across the cell wall. We lastly propose a distinct architecture for conjugative T4SSs in Gram-positive bacteria compared to their Gram-negative counterparts, possibly attributed to the differences in the cell wall structure.

Elucidating assembly and function of VirB8 cell wall subunits refines the DNA translocation model in Gram-positive T4SSs.,Maffo-Woulefack R, Ali AM, Laroussi H, Cappele J, Romero-Saavedra F, Ramia N, Robert E, Mathiot S, Soler N, Roussel Y, Fronzes R, Huebner J, Didierjean C, Favier F, Leblond-Bourget N, Douzi B Sci Adv. 2025 Jan 24;11(4):eadq5975. doi: 10.1126/sciadv.adq5975. Epub 2025 Jan , 22. PMID:39841841<ref>PMID:39841841</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7pkw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus thermophilus]]
[[Category: Cappele J]]
[[Category: Didierjean C]]
[[Category: Douzi B]]
[[Category: Favier F]]
[[Category: Leblond-Bourget N]]