View source for 7plx


==Cryo-EM structure of the actomyosin-V complex in the rigor state (central 1er, class 4)==
<StructureSection load='7plx' size='340' side='right'caption='[[7plx]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7plx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Amanita_phalloides Amanita phalloides], [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PLX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DTH:D-THREONINE'>DTH</scene>, <scene name='pdbligand=EEP:(2S,4R)-2-amino-4,5-dihydroxy-4-methylpentanoic+acid+(non-preferred+name)'>EEP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7plx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7plx OCA], [https://pdbe.org/7plx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7plx RCSB], [https://www.ebi.ac.uk/pdbsum/7plx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7plx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MYO5A_CHICK MYO5A_CHICK] Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The molecular motor myosin undergoes a series of major structural transitions during its force-producing motor cycle. The underlying mechanism and its coupling to ATP hydrolysis and actin binding is only partially understood, mostly due to sparse structural data on actin-bound states of myosin. Here, we report 26 high-resolution cryo-EM structures of the actomyosin-V complex in the strong-ADP, rigor, and a previously unseen post-rigor transition state that binds the ATP analog AppNHp. The structures reveal a high flexibility of myosin in each state and provide valuable insights into the structural transitions of myosin-V upon ADP release and binding of AppNHp, as well as the actomyosin interface. In addition, they show how myosin is able to specifically alter the structure of F-actin.

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.,Pospich S, Sweeney HL, Houdusse A, Raunser S Elife. 2021 Nov 23;10. pii: 73724. doi: 10.7554/eLife.73724. PMID:34812732<ref>PMID:34812732</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7plx" style="background-color:#fffaf0;"></div>

==See Also==
*[[Actin 3D structures|Actin 3D structures]]
*[[Myosin 3D Structures|Myosin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Amanita phalloides]]
[[Category: Gallus gallus]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Houdusse A]]
[[Category: Pospich S]]
[[Category: Raunser S]]
[[Category: Sweeney HL]]