View source for 7ppz


==Crystal structure of the Burkholderia Lethal Factor 1 (BLF1) C94S inactive mutant in complex with human eIF4A - Crystal form A==
<StructureSection load='7ppz' size='340' side='right'caption='[[7ppz]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ppz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PPZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ppz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ppz OCA], [https://pdbe.org/7ppz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ppz RCSB], [https://www.ebi.ac.uk/pdbsum/7ppz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ppz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q63UP7_BURPS Q63UP7_BURPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Burkholderia pseudomallei lethal factor 1 (BLF1) exhibits site-specific glutamine deamidase activity against the eukaryotic RNA helicase, eIF4A, thereby blocking mammalian protein synthesis. The structure of a complex between BLF1 C94S and human eIF4A shows that the toxin binds in the cleft between the two RecA-like eIF4A domains forming interactions with residues from both and with the scissile amide of the target glutamine, Gln339, adjacent to the toxin active site. The RecA-like domains adopt a radically twisted orientation compared to other eIF4A structures and the nature and position of conserved residues suggests this may represent a conformation associated with RNA binding. Comparison of the catalytic site of BLF1 with other deamidases and cysteine proteases reveals that they fall into two classes, related by pseudosymmetry, that present either the re or si faces of the target amide/peptide to the nucleophilic sulfur, highlighting constraints in the convergent evolution of their Cys-His active sites.

Molecular basis of specificity and deamidation of eIF4A by Burkholderia Lethal Factor 1.,Mobbs GW, Aziz AA, Dix SR, Blackburn GM, Sedelnikova SE, Minshull TC, Dickman MJ, Baker PJ, Nathan S, Raih MF, Rice DW Commun Biol. 2022 Mar 28;5(1):272. doi: 10.1038/s42003-022-03186-2. PMID:35347220<ref>PMID:35347220</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ppz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Burkholderia pseudomallei K96243]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Aziz AA]]
[[Category: Baker PJ]]
[[Category: Blackburn GM]]
[[Category: Dickman MJ]]
[[Category: Dix SR]]
[[Category: Firdaus-Raih M]]
[[Category: Minshull TC]]
[[Category: Mobbs GW]]
[[Category: Nathan S]]
[[Category: Rice DW]]
[[Category: Sedelnikova SE]]