View source for 7pr5


==Cocrystal of an RSL-N23H and sulfonato-thiacalix[4]arene - zinc complex==
<StructureSection load='7pr5' size='340' side='right'caption='[[7pr5]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7pr5]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PR5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=80M:sulfonato-thiacalix[4]arene'>80M</scene>, <scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pr5 OCA], [https://pdbe.org/7pr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pr5 RCSB], [https://www.ebi.ac.uk/pdbsum/7pr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pr5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Controlled protein assembly provides a means to generate biomaterials. Synthetic macrocycles such as the water-soluble sulfonato-calix[n]arenes are useful mediators of protein assembly. Sulfonato-thiacalix[4]arene (tsclx 4 ), with its metal-binding capacity, affords the potential for simultaneous macrocycle- and metal-mediated protein assembly. Here, we describe the tsclx 4 -/Zn-directed assembly of two proteins: cationic alpha-helical cytochrome c (cyt c) and neutral beta-propeller Ralstonia solanacearum lectin (RSL). Two co-crystal forms were obtained with cyt c, each involving multinuclear zinc sites supported by the cone conformation of tsclx 4 . The tsclx 4 /Zn cluster acted as an assembly node via both lysine encapsulation and metal-mediated protein-protein contacts. In the case of RSL, tsclx 4 adopted the 1,2-alternate conformation and supported a dinuclear zinc site with concomitant encapsulation and metal-binding of two histidine side chains. These results, together with the knowledge of thiacalixarene/metal nanoclusters, suggest promising applications for thiacalixarenes in biomaterials and MOF fabrication.

Protein Frameworks with Thiacalixarene and Zinc.,Flood RJ, Ramberg KO, Mengel DB, Guagnini F, Crowley PB Cryst Growth Des. 2022 May 4;22(5):3271-3276. doi: 10.1021/acs.cgd.2c00108. Epub , 2022 Feb 22. PMID:35529063<ref>PMID:35529063</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7pr5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ralstonia solanacearum]]
[[Category: Crowley PB]]
[[Category: Flood RJ]]
[[Category: Guagnini F]]
[[Category: Ramberg K]]