View source for 7q22


==cryo iDPC-STEM structure recorded with CSA 2.0==
<StructureSection load='7q22' size='340' side='right'caption='[[7q22]], [[Resolution|resolution]] 6.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7q22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus_(vulgare) Tobacco mosaic virus (vulgare)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q22 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q22 OCA], [https://pdbe.org/7q22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q22 RCSB], [https://www.ebi.ac.uk/pdbsum/7q22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q22 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_TMV CAPSD_TMV] Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron microscopy (STEM) using the integrated differential phase contrast mode also known as iDPC-STEM to two cryo-EM test specimens, keyhole limpet hemocyanin (KLH) and tobacco mosaic virus (TMV). The micrographs show complete contrast transfer to high resolution and enable the cryo-EM structure determination for KLH at 6.5 A resolution, as well as for TMV at 3.5 A resolution using single-particle reconstruction methods, which share identical features with maps obtained by CTEM of a previously acquired same-sized TMV data set. These data show that STEM imaging in general, and in particular the iDPC-STEM approach, can be applied to vitrified single-particle specimens to determine near-atomic resolution cryo-EM structures of biological macromolecules.

Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.,Lazic I, Wirix M, Leidl ML, de Haas F, Mann D, Beckers M, Pechnikova EV, Muller-Caspary K, Egoavil R, Bosch EGT, Sachse C Nat Methods. 2022 Sep;19(9):1126-1136. doi: 10.1038/s41592-022-01586-0. Epub 2022 , Sep 5. PMID:36064775<ref>PMID:36064775</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7q22" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leidl ML]]
[[Category: Sachse C]]