View source for 7qgr


==Structure of the SmrB-bound E. coli disome - collided 70S ribosome==
<StructureSection load='7qgr' size='340' side='right'caption='[[7qgr]], [[Resolution|resolution]] 5.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qgr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QGR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qgr OCA], [https://pdbe.org/7qgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qgr RCSB], [https://www.ebi.ac.uk/pdbsum/7qgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qgr ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Ribosome rescue pathways recycle stalled ribosomes and target problematic mRNAs and aborted proteins for degradation(1,2). In bacteria, it remains unclear how rescue pathways distinguish ribosomes stalled in the middle of a transcript from actively translating ribosomes(3-6). Here, using a genetic screen in Escherichia coli, we discovered a new rescue factor that has endonuclease activity. SmrB cleaves mRNAs upstream of stalled ribosomes, allowing the ribosome rescue factor tmRNA (which acts on truncated mRNAs(3)) to rescue upstream ribosomes. SmrB is recruited to ribosomes and is activated by collisions. Cryo-electron microscopy structures of collided disomes from E. coli and Bacillus subtilis show distinct and conserved arrangements of individual ribosomes and the composite SmrB-binding site. These findings reveal the underlying mechanisms by which ribosome collisions trigger ribosome rescue in bacteria.

Ribosome collisions induce mRNA cleavage and ribosome rescue in bacteria.,Saito K, Kratzat H, Campbell A, Buschauer R, Burroughs AM, Berninghausen O, Aravind L, Green R, Beckmann R, Buskirk AR Nature. 2022 Mar;603(7901):503-508. doi: 10.1038/s41586-022-04416-7. Epub 2022 , Mar 9. PMID:35264790<ref>PMID:35264790</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7qgr" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Beckmann R]]
[[Category: Berninghausen O]]
[[Category: Buschauer R]]
[[Category: Kratzat H]]