View source for 7qje


==Structure of recombinant human gamma-Tubulin Ring Complex 4-spoked assembly intermediate (spokes 9-12)==
<StructureSection load='7qje' size='340' side='right'caption='[[7qje]], [[Resolution|resolution]] 7.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qje]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QJE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qje OCA], [https://pdbe.org/7qje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qje RCSB], [https://www.ebi.ac.uk/pdbsum/7qje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qje ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCP4_HUMAN GCP4_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The gamma-tubulin ring complex (gamma-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the gamma-TuRC, containing more than thirty subunits, raising fundamental questions about gamma-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular gamma-TuRC assembly and identify a functional role of actin in microtubule nucleation. During gamma-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the gamma-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for gamma-TuRC assembly and structural integrity, but determines gamma-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo.

Modular assembly of the principal microtubule nucleator gamma-TuRC.,Wurtz M, Zupa E, Atorino ES, Neuner A, Bohler A, Rahadian AS, Vermeulen BJA, Tonon G, Eustermann S, Schiebel E, Pfeffer S Nat Commun. 2022 Jan 25;13(1):473. doi: 10.1038/s41467-022-28079-0. PMID:35078983<ref>PMID:35078983</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qje" style="background-color:#fffaf0;"></div>

==See Also==
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Pfeffer S]]
[[Category: Tonon G]]
[[Category: Zupa E]]