View source for 7qjj


==X-Ray Structure of a Mn2+ soak of EleNRMT in complex with two Nanobodies at 4.6A==
<StructureSection load='7qjj' size='340' side='right'caption='[[7qjj]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qjj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QJJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qjj OCA], [https://pdbe.org/7qjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qjj RCSB], [https://www.ebi.ac.uk/pdbsum/7qjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qjj ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Members of the ubiquitous SLC11/NRAMP family catalyze the uptake of divalent transition metal ions into cells. They have evolved to efficiently select these trace elements from a large pool of Ca(2+) and Mg(2+), which are both orders of magnitude more abundant, and to concentrate them in the cytoplasm aided by the cotransport of H(+) serving as energy source. In the present study, we have characterized a member of a distant clade of the family found in prokaryotes, termed NRMTs, that were proposed to function as transporters of Mg(2+). The protein transports Mg(2+) and Mn(2+) but not Ca(2+) by a mechanism that is not coupled to H(+). Structures determined by cryo-EM and X-ray crystallography revealed a generally similar protein architecture compared to classical NRAMPs, with a restructured ion binding site whose increased volume provides suitable interactions with ions that likely have retained much of their hydration shell.

Structural and functional properties of a magnesium transporter of the SLC11/NRAMP family.,Ramanadane K, Straub MS, Dutzler R, Manatschal C Elife. 2022 Jan 10;11:e74589. doi: 10.7554/eLife.74589. PMID:35001872<ref>PMID:35001872</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qjj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vicugna pacos]]
[[Category: Dutzler R]]
[[Category: Manatschal C]]
[[Category: Ramanadane K]]
[[Category: Straub MS]]