View source for 7qok


==Tail muzzle assembly of the phicrAss001 virion with C6 symmetry imposed==
<StructureSection load='7qok' size='340' side='right'caption='[[7qok]], [[Resolution|resolution]] 3.38&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qok]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/CrAss-like_viruses CrAss-like viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.38&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qok OCA], [https://pdbe.org/7qok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qok RCSB], [https://www.ebi.ac.uk/pdbsum/7qok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qok ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MUZZL_BPCA1 MUZZL_BPCA1] Forms the muzzle assembly at the distal part of the tail. May serve as the gatekeeper to control the ejection of the cargo proteins.<ref>PMID:37138077</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CrAssphage and related viruses of the order Crassvirales (hereafter referred to as crassviruses) were originally discovered by cross-assembly of metagenomic sequences. They are the most abundant viruses in the human gut, are found in the majority of individual gut viromes, and account for up to 95% of the viral sequences in some individuals(1-4). Crassviruses are likely to have major roles in shaping the composition and functionality of the human microbiome, but the structures and roles of most of the virally encoded proteins are unknown, with only generic predictions resulting from bioinformatic analyses(4,5). Here we present a cryo-electron microscopy reconstruction of Bacteroides intestinalis virus PhicrAss001(6), providing the structural basis for the functional assignment of most of its virion proteins. The muzzle protein forms an assembly about 1 MDa in size at the end of the tail and exhibits a previously unknown fold that we designate the 'crass fold', that is likely to serve as a gatekeeper that controls the ejection of cargos. In addition to packing the approximately 103 kb of virus DNA, the PhicrAss001 virion has extensive storage space for virally encoded cargo proteins in the capsid and, unusually, within the tail. One of the cargo proteins is present in both the capsid and the tail, suggesting a general mechanism for protein ejection, which involves partial unfolding of proteins during their extrusion through the tail. These findings provide a structural basis for understanding the mechanisms of assembly and infection of these highly abundant crassviruses.

Structural atlas of a human gut crassvirus.,Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA Nature. 2023 May;617(7960):409-416. doi: 10.1038/s41586-023-06019-2. Epub 2023 , May 3. PMID:37138077<ref>PMID:37138077</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qok" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: CrAss-like viruses]]
[[Category: Large Structures]]
[[Category: Antson AA]]
[[Category: Bayfield OW]]
[[Category: Hawkins DEDP]]
[[Category: Hill C]]
[[Category: Khokhlova EV]]
[[Category: Koonin EV]]
[[Category: Shkoporov AN]]
[[Category: Smith JLR]]
[[Category: Yutin N]]