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==Structure of the human inner kinetochore CCAN complex==
<StructureSection load='7qoo' size='340' side='right'caption='[[7qoo]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qoo]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QOO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QOO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qoo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qoo OCA], [https://pdbe.org/7qoo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qoo RCSB], [https://www.ebi.ac.uk/pdbsum/7qoo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qoo ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CENPK_HUMAN CENPK_HUMAN] Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with KMT2A/MLL1.<ref>PMID:8950979</ref> 
== Function ==
[https://www.uniprot.org/uniprot/CENPK_HUMAN CENPK_HUMAN] Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.<ref>PMID:16622420</ref> <ref>PMID:16716197</ref> <ref>PMID:18045986</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Centromeres are specialized chromosome loci that seed the kinetochore, a large protein complex that effects chromosome segregation. A 16-subunit complex, the constitutive centromere associated network (CCAN), connects between the specialized centromeric chromatin, marked by the histone H3 variant CENP-A, and the spindle-binding moiety of the kinetochore. Here, we report a cryo-electron microscopy structure of human CCAN. We highlight unique features such as the pseudo GTPase CENP-M and report how a crucial CENP-C motif binds the CENP-LN complex. The CCAN structure has implications for the mechanism of specific recognition of the CENP-A nucleosome. A model consistent with our structure depicts the CENP-C-bound nucleosome as connected to the CCAN through extended, flexible regions of CENP-C. An alternative model identifies both CENP-C and CENP-N as specificity determinants but requires CENP-N to bind CENP-A in a mode distinct from the classical nucleosome octamer.

Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization.,Pesenti ME, Raisch T, Conti D, Walstein K, Hoffmann I, Vogt D, Prumbaum D, Vetter IR, Raunser S, Musacchio A Mol Cell. 2022 Jun 2;82(11):2113-2131.e8. doi: 10.1016/j.molcel.2022.04.027. Epub , 2022 May 6. PMID:35525244<ref>PMID:35525244</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qoo" style="background-color:#fffaf0;"></div>

==See Also==
*[[Centromere protein 3D structure|Centromere protein 3D structure]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Pesenti M]]
[[Category: Raisch T]]
[[Category: Vetter IR]]