View source for 7qpg


==Human RZZ kinetochore corona complex.==
<StructureSection load='7qpg' size='340' side='right'caption='[[7qpg]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qpg]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QPG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qpg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qpg OCA], [https://pdbe.org/7qpg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qpg RCSB], [https://www.ebi.ac.uk/pdbsum/7qpg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qpg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZWILC_HUMAN ZWILC_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In metazoans, a approximately 1 megadalton (MDa) multiprotein complex comprising the dynein-dynactin adaptor Spindly and the ROD-Zwilch-ZW10 (RZZ) complex is the building block of a fibrous biopolymer, the kinetochore fibrous corona. The corona assembles on mitotic kinetochores to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. We report here a high-resolution cryo-EM structure that captures the essential features of the RZZ complex, including a farnesyl-binding site required for Spindly binding. Using a highly predictive in vitro assay, we demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly (RZZS) complex, and describe the molecular mechanism of phosphorylation-dependent filament nucleation. We identify several structural requirements for RZZS polymerization in rings and sheets. Finally, we identify determinants of kinetochore localization and corona assembly of Spindly. Our results describe a framework for the long-sought-for molecular basis of corona assembly on metazoan kinetochores.

Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores.,Raisch T, Ciossani G, d'Amico E, Cmentowski V, Carmignani S, Maffini S, Merino F, Wohlgemuth S, Vetter IR, Raunser S, Musacchio A EMBO J. 2022 May 2;41(9):e110411. doi: 10.15252/embj.2021110411. Epub 2022 Apr 4. PMID:35373361<ref>PMID:35373361</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qpg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Carmignani S]]
[[Category: Ciossani G]]
[[Category: Cmetowski V]]
[[Category: Maffini S]]
[[Category: Merino F]]
[[Category: Musacchio A]]
[[Category: Raisch T]]
[[Category: Raunser S]]
[[Category: Vetter IR]]
[[Category: Wohlgemuth S]]
[[Category: D'Amico E]]