View source for 7qqm


==The PDZ domain of LRRC7 fused with ANXA2==
<StructureSection load='7qqm' size='340' side='right'caption='[[7qqm]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QQM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qqm OCA], [https://pdbe.org/7qqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qqm RCSB], [https://www.ebi.ac.uk/pdbsum/7qqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qqm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LRRC7_HUMAN LRRC7_HUMAN] Required for normal synaptic spine architecture and function. Necessary for DISC1 and GRM5 localization to postsynaptic density complexes and for both N-methyl D-aspartate receptor-dependent and metabotropic glutamate receptor-dependent long term depression.<ref>PMID:11729199</ref> [https://www.uniprot.org/uniprot/ANXA2_HUMAN ANXA2_HUMAN] Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally solved structures and most of their complexes remain to be solved. Here, a simple and cost-effective strategy is proposed for the crystallization of PDZ domains and their complexes. A human annexin A2 fusion tag was used as a crystallization chaperone and the structures of nine PDZ domains were solved, including five domains that had not yet been solved. Finally, these novel experimental structures were compared with AlphaFold predictions and it is speculated how predictions and experimental methods could cooperate in order to investigate the structural landscapes of entire domain families and interactomes.

A scalable strategy to solve structures of PDZ domains and their complexes.,Cousido-Siah A, Carneiro L, Kostmann C, Ecsedi P, Nyitray L, Trave G, Gogl G Acta Crystallogr D Struct Biol. 2022 Apr 1;78(Pt 4):509-516. doi:, 10.1107/S2059798322001784. Epub 2022 Mar 11. PMID:35362473<ref>PMID:35362473</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qqm" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Cousido-Siah A]]
[[Category: Gogl G]]
[[Category: Trave G]]