View source for 7qxi


==Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex==
<StructureSection load='7qxi' size='340' side='right'caption='[[7qxi]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qxi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QXI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qxi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qxi OCA], [https://pdbe.org/7qxi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qxi RCSB], [https://www.ebi.ac.uk/pdbsum/7qxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qxi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RPOC_ECOLI RPOC_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01322]
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== Publication Abstract from PubMed ==
Gene transcription is carried out by RNA polymerase (RNAP) and requires the conversion of the initial closed promoter complex, where DNA is double stranded, to a transcription-competent open promoter complex, where DNA is opened up. In bacteria, RNAP relies on sigma factors for its promoter specificities. Using a special form of sigma factor (sigma(54)), which forms a stable closed complex and requires its activator that belongs to the AAA+ ATPases (ATPases associated with diverse cellular activities), we obtained cryo-electron microscopy structures of transcription initiation complexes that reveal a previously unidentified process of DNA melting opening. The sigma(54) amino terminus threads through the locally opened up DNA and then becomes enclosed by the AAA+ hexameric ring in the activator-bound intermediate complex. Our structures suggest how ATP hydrolysis by the AAA+ activator could remove the sigma(54) inhibition while helping to open up DNA, using sigma(54) amino-terminal peptide as a pry bar.

Mechanisms of DNA opening revealed in AAA+ transcription complex structures.,Ye F, Gao F, Liu X, Buck M, Zhang X Sci Adv. 2022 Dec 21;8(51):eadd3479. doi: 10.1126/sciadv.add3479. Epub 2022 Dec , 21. PMID:36542713<ref>PMID:36542713</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7qxi" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Ye FZ]]
[[Category: Zhang XD]]