View source for 7qzd


==Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikF with an engineered HMA domain of Pikp-1 (Pikp-SNK-EKE) from rice (Oryza sativa)==
<StructureSection load='7qzd' size='340' side='right'caption='[[7qzd]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qzd]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group] and [https://en.wikipedia.org/wiki/Pyricularia_oryzae Pyricularia oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QZD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QZD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qzd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qzd OCA], [https://pdbe.org/7qzd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qzd RCSB], [https://www.ebi.ac.uk/pdbsum/7qzd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qzd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D5L9G5_ORYSJ D5L9G5_ORYSJ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by phytopathogens to promote infection, through unconventional integrated domains which resemble the effector's host targets. Direct binding of effectors to these integrated domains activates plant defenses. The rice NLR receptor Pik-1 binds the Magnaporthe oryzae effector AVR-Pik through an integrated heavy metal-associated (HMA) domain. However, the stealthy alleles AVR-PikC and AVR-PikF avoid interaction with Pik-HMA and evade host defenses. Here, we exploited knowledge of the biochemical interactions between AVR-Pik and its host target, OsHIPP19, to engineer novel Pik-1 variants that respond to AVR-PikC/F. First, we exchanged the HMA domain of Pikp-1 for OsHIPP19-HMA, demonstrating that effector targets can be incorporated into NLR receptors to provide novel recognition profiles. Second, we used the structure of OsHIPP19-HMA to guide the mutagenesis of Pikp-HMA to expand its recognition profile. We demonstrate that the extended recognition profiles of engineered Pikp-1 variants correlate with effector binding in planta and in vitro, and with the gain of new contacts across the effector/HMA interface. Crucially, transgenic rice producing the engineered Pikp-1 variants was resistant to blast fungus isolates carrying AVR-PikC or AVR-PikF. These results demonstrate that effector target-guided engineering of NLR receptors can provide new-to-nature disease resistance in crops.

Effector target-guided engineering of an integrated domain expands the disease resistance profile of a rice NLR immune receptor.,Maidment JHR, Shimizu M, Bentham AR, Vera S, Franceschetti M, Longya A, Stevenson CEM, De la Concepcion JC, Bialas A, Kamoun S, Terauchi R, Banfield MJ Elife. 2023 May 18;12:e81123. doi: 10.7554/eLife.81123. PMID:37199729<ref>PMID:37199729</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qzd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Pyricularia oryzae]]
[[Category: Banfield MJ]]
[[Category: Franceschetti M]]
[[Category: Longya A]]
[[Category: Maidment JHR]]