View source for 7roa


==Crystal structure of EntV136 from Enterococcus faecalis==
<StructureSection load='7roa' size='340' side='right'caption='[[7roa]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7roa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_OG1RF Enterococcus faecalis OG1RF]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ROA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ROA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7roa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7roa OCA], [https://pdbe.org/7roa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7roa RCSB], [https://www.ebi.ac.uk/pdbsum/7roa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7roa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q836L4_ENTFA Q836L4_ENTFA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fungal pathogens are a continuing challenge due to few effective antifungals and a rise in resistance. In previous work, we described the inhibition of Candida albicans virulence following exposure to the 68 amino acid bacteriocin, EntV, secreted by Enterococcus faecalis. Here, to optimize EntV as a potential therapeutic and better understand its antifungal features, an X-ray structure is obtained. The structure consists of six alpha helices enclosing a seventh 16 amino acid helix (alpha7). The individual helices are tested for antifungal activity using in vitro and nematode infection assays. Interestingly, alpha7 retains antifungal, but not antibacterial activity and is also effective against Candida auris and Cryptococcus neoformans. Further reduction of alpha7 to 12 amino acids retains full antifungal activity, and excellent efficacy is observed in rodent models of C. albicans oropharyngeal, systemic, and venous catheter infections. Together, these results showcase EntV-derived peptides as promising candidates for antifungal therapeutic development.

Structural and functional analysis of EntV reveals a 12 amino acid fragment protective against fungal infections.,Cruz MR, Cristy S, Guha S, De Cesare GB, Evdokimova E, Sanchez H, Borek D, Miramon P, Yano J, Fidel PL Jr, Savchenko A, Andes DR, Stogios PJ, Lorenz MC, Garsin DA Nat Commun. 2022 Oct 13;13(1):6047. doi: 10.1038/s41467-022-33613-1. PMID:36229448<ref>PMID:36229448</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7roa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterococcus faecalis OG1RF]]
[[Category: Large Structures]]
[[Category: Evdokimova E]]
[[Category: Garsin D]]
[[Category: Joachimiak A]]
[[Category: Kim Y]]
[[Category: Satchell KJF]]
[[Category: Savchenko A]]
[[Category: Stogios PJ]]