View source for 7rt7


==Crystal structure of the RhsP2 C-terminal toxin domain in complex with its immunity protein, RhsI2==
<StructureSection load='7rt7' size='340' side='right'caption='[[7rt7]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7rt7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RT7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rt7 OCA], [https://pdbe.org/7rt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rt7 RCSB], [https://www.ebi.ac.uk/pdbsum/7rt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rt7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0H2Z8A2_PSEAB A0A0H2Z8A2_PSEAB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ADP-ribosyltransferases (ARTs) were among the first identified bacterial virulence factors. Canonical ART toxins are delivered into host cells where they modify essential proteins, thereby inactivating cellular processes and promoting pathogenesis. Our understanding of ARTs has since expanded beyond protein-targeting toxins to include antibiotic inactivation and DNA damage repair. Here, we report the discovery of RhsP2 as an ART toxin delivered between competing bacteria by a type VI secretion system of Pseudomonas aeruginosa. A structure of RhsP2 reveals that it resembles protein-targeting ARTs such as diphtheria toxin. Remarkably, however, RhsP2 ADP-ribosylates 2'-hydroxyl groups of double-stranded RNA, and thus, its activity is highly promiscuous with identified cellular targets including the tRNA pool and the RNA-processing ribozyme, ribonuclease P. Consequently, cell death arises from the inhibition of translation and disruption of tRNA processing. Overall, our data demonstrate a previously undescribed mechanism of bacterial antagonism and uncover an unprecedented activity catalyzed by ART enzymes.

An ADP-ribosyltransferase toxin kills bacterial cells by modifying structured non-coding RNAs.,Bullen NP, Sychantha D, Thang SS, Culviner PH, Rudzite M, Ahmad S, Shah VS, Filloux A, Prehna G, Whitney JC Mol Cell. 2022 Sep 15;82(18):3484-3498.e11. doi: 10.1016/j.molcel.2022.08.015. , Epub 2022 Sep 6. PMID:36070765<ref>PMID:36070765</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7rt7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
[[Category: Bullen NP]]
[[Category: Prehna G]]
[[Category: Whitney JC]]