View source for 7rty


==Crystal Structure of PsfC from Pseudomonas syringae PB-5123==
<StructureSection load='7rty' size='340' side='right'caption='[[7rty]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7rty]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RTY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.963&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rty OCA], [https://pdbe.org/7rty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rty RCSB], [https://www.ebi.ac.uk/pdbsum/7rty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rty ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I6QY72_PSESX I6QY72_PSESX] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The epoxide-containing phosphonate natural product fosfomycin is a broad-spectrum antibiotic used in the treatment of cystitis. Fosfomycin is produced by both the plant pathogen Pseudomonas syringae and soil-dwelling streptomycetes. While the streptomycete pathway has recently been fully elucidated, the pseudomonad pathway is still mostly elusive. Through a systematic evaluation of heterologous expression of putative biosynthetic enzymes, we identified the central enzyme responsible for completing the biosynthetic pathway in pseudomonads. The missing transformation involves the oxidative decarboxylation of the intermediate 2-phosphonomethylmalate to a new intermediate, 3-oxo-4-phosphonobutanoate, by PsfC. Crystallographic studies reveal that PsfC unexpectedly belongs to a new class of diiron metalloenzymes that are part of the polymerase and histidinol phosphatase superfamily.

Biosynthesis of fosfomycin in pseudomonads reveals an unexpected enzymatic activity in the metallohydrolase superfamily.,Simon MA, Ongpipattanakul C, Nair SK, van der Donk WA Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2019863118. doi:, 10.1073/pnas.2019863118. PMID:34074759<ref>PMID:34074759</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7rty" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas syringae]]
[[Category: Nair SK]]
[[Category: Ongpipattanakul C]]