View source for 7rws


==Structure of SAVED domain of Cap5 from Lactococcus lactis in complex with cGAMP==
<StructureSection load='7rws' size='340' side='right'caption='[[7rws]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7rws]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris_IBB477 Lactococcus lactis subsp. cremoris IBB477]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RWS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4UR:32-CGAMP'>4UR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rws OCA], [https://pdbe.org/7rws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rws RCSB], [https://www.ebi.ac.uk/pdbsum/7rws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rws ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1E7G0A2_LACLC A0A1E7G0A2_LACLC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cyclic-oligonucleotide-based antiphage signaling systems (CBASS) are diverse and abundant in bacteria. Here, we present the biochemical and structural characterization of two CBASS systems, composed of CdnG and Cap5, from Asticcacaulis sp. and Lactococcus lactis. We show that CdnG from Asticcacaulis sp. synthesizes 3',2'-cGAMP in vitro, and 3',2'-cGAMP is the biological signaling molecule that activates Cap5 for DNA degradation. Crystal structures of Cap5, together with the SAVED domain in complex with 3',2'-cGAMP, provide insight into the architecture of Cap5 as well as molecular recognition of 3',2'-cGAMP by the SAVED domain of Cap5. Amino acid conservation of the SAVED domain of Cap5, together with mutational studies, led us to propose a mechanism of Back-to-Front stacking of two SAVED domains, mediated by 3',2'-cGAMP, to activate HNH nuclease domain for DNA degradation. This study of the most abundant CBASS system provides insights into the mechanisms employed by bacteria in their conflicts against phage.

Molecular mechanisms of the CdnG-Cap5 antiphage defense system employing 3',2'-cGAMP as the second messenger.,Fatma S, Chakravarti A, Zeng X, Huang RH Nat Commun. 2021 Nov 4;12(1):6381. doi: 10.1038/s41467-021-26738-2. PMID:34737303<ref>PMID:34737303</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7rws" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Lactococcus lactis subsp. cremoris IBB477]]
[[Category: Large Structures]]
[[Category: Chakravarti A]]
[[Category: Fatma S]]
[[Category: Huang RH]]