View source for 7rwt


==Adeno-associated virus type 2==
<StructureSection load='7rwt' size='340' side='right'caption='[[7rwt]], [[Resolution|resolution]] 2.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RWT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.43&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rwt OCA], [https://pdbe.org/7rwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rwt RCSB], [https://www.ebi.ac.uk/pdbsum/7rwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rwt ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 A resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity.

Structural characterization of an envelope-associated adeno-associated virus type 2 capsid.,Hull JA, Mietzsch M, Chipman P, Strugatsky D, McKenna R Virology. 2021 Oct 6;565:22-28. doi: 10.1016/j.virol.2021.09.010. PMID:34638006<ref>PMID:34638006</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7rwt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chipman P]]
[[Category: Hull JA]]
[[Category: McKenna R]]
[[Category: Mietzsch M]]
[[Category: Strugatsky D]]