View source for 7s4h


==CryoEM structure of Methylococcus capsulatus (Bath) pMMO in a native lipid nanodisc at 2.14 Angstrom resolution==
<StructureSection load='7s4h' size='340' side='right'caption='[[7s4h]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7s4h]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylococcus_capsulatus_str._Bath Methylococcus capsulatus str. Bath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S4H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S4H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.14&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=HXG:1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>HXG</scene>, <scene name='pdbligand=P1O:1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>P1O</scene>, <scene name='pdbligand=PLC:DIUNDECYL+PHOSPHATIDYL+CHOLINE'>PLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s4h OCA], [https://pdbe.org/7s4h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s4h RCSB], [https://www.ebi.ac.uk/pdbsum/7s4h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s4h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PMOB_METCA PMOB_METCA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial methane oxidation using the enzyme particulate methane monooxygenase (pMMO) contributes to the removal of environmental methane, a potent greenhouse gas. Crystal structures determined using inactive, detergent-solubilized pMMO lack several conserved regions neighboring the proposed active site. We show that reconstituting pMMO in nanodiscs with lipids extracted from the native organism restores methane oxidation activity. Multiple nanodisc-embedded pMMO structures determined by cryo-electron microscopy to 2.14- to 2.46-angstrom resolution reveal the structure of pMMO in a lipid environment. The resulting model includes stabilizing lipids, regions of the PmoA and PmoC subunits not observed in prior structures, and a previously undetected copper-binding site in the PmoC subunit with an adjacent hydrophobic cavity. These structures provide a revised framework for understanding and engineering pMMO function.

Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer.,Koo CW, Tucci FJ, He Y, Rosenzweig AC Science. 2022 Mar 18;375(6586):1287-1291. doi: 10.1126/science.abm3282. Epub 2022 , Mar 17. PMID:35298269<ref>PMID:35298269</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7s4h" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methane monooxygenase 3D structures|Methane monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylococcus capsulatus str. Bath]]
[[Category: Koo CW]]
[[Category: Rosenzweig AC]]