View source for 7sag


==Solution NMR structure of barrettide C==
<StructureSection load='7sag' size='340' side='right'caption='[[7sag]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sag OCA], [https://pdbe.org/7sag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sag RCSB], [https://www.ebi.ac.uk/pdbsum/7sag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sag ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Natural product discovery by isolation and structure elucidation is a laborious task often requiring ample quantities of biological starting material and frequently resulting in the rediscovery of previously known compounds. However, peptides are a compound class amenable to an alternative genomic, transcriptomic, and in silico discovery route by similarity searches of known peptide sequences against sequencing data. Based on the sequences of barrettides A and B, we identified five new barrettide sequences (barrettides C-G) predicted from the North Atlantic deep-sea demosponge Geodia barretti (Geodiidae). We synthesized, folded, and investigated one of the newly described barrettides, barrettide C (NVVPCFCVEDETSGAKTCIPDNCDASRGTNP, disulfide connectivity I-IV, II-III). Co-elution experiments of synthetic and sponge-derived barrettide C confirmed its native conformation. NMR spectroscopy and the anti-biofouling activity on larval settlement of the bay barnacle Amphibalanus improvisus (IC50 0.64 muM) show that barrettide C is highly similar to barrettides A and B in both structure and function. Several lines of evidence suggest that barrettides are produced by the sponge itself and not one of its microbial symbionts.

Barrettides: A Peptide Family Specifically Produced by the Deep-Sea Sponge Geodia barretti.,Steffen K, Laborde Q, Gunasekera S, Payne CD, Rosengren KJ, Riesgo A, Goransson U, Cardenas P J Nat Prod. 2021 Dec 24;84(12):3138-3146. doi: 10.1021/acs.jnatprod.1c00938. Epub, 2021 Dec 7. PMID:34874154<ref>PMID:34874154</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Payne CD]]
[[Category: Rosengren KJ]]