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==Mu-conotoxin KIIIA isomer 2==
<StructureSection load='7saw' size='340' side='right'caption='[[7saw]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7saw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7saw OCA], [https://pdbe.org/7saw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7saw RCSB], [https://www.ebi.ac.uk/pdbsum/7saw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7saw ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
mu-Conotoxins are components of cone snail venom, well-known for their analgesic activity through potent inhibition of voltage-gated sodium channel (NaV) subtypes, including NaV1.7. These small, disulfide-rich peptides are typically stabilized by three disulfide bonds arranged in a 'native' CysI-CysIV, CysII-CysV, CysIII-CysVI pattern of disulfide connectivity. However, mu-conotoxin KIIIA, the smallest and most studied mu-conotoxin with inhibitory activity at NaV1.7, forms two distinct disulfide bond isomers during thermodynamic oxidative folding, including Isomer 1 (CysI-CysV, CysII-CysIV, CysIII-CysVI) and Isomer 2 (CysI-CysVI, CysII-CysIV, CysIII-CysV), but not the native mu-conotoxin arrangement. To date, there has been no study on the structure and activity of KIIIA comprising the native mu-conotoxin disulfide bond arrangement. Here, we evaluated the synthesis, potency, sodium channel subtype selectivity, and 3D structure of the three isomers of KIIIA. Using a regioselective disulfide bond-forming strategy, we synthetically produced the three mu-conotoxin KIIIA isomers displaying distinct bioactivity and NaV subtype selectivity across human NaV channel subtypes 1.2, 1.4, and 1.7. We show that Isomer 1 inhibits NaV subtypes with a rank order of potency of NaV1.4 &gt; 1.2 &gt; 1.7 and Isomer 2 in the order of NaV1.4 approximately 1.2 &gt; 1.7, while the native isomer inhibited NaV1.4 &gt; 1.7 approximately 1.2. The three KIIIA isomers were further evaluated by NMR solution structure analysis and molecular docking with hNaV1.2. Our study highlights the importance of investigating alternate disulfide isomers, as disulfide connectivity affects not only the overall structure of the peptides but also the potency and subtype selectivity of mu-conotoxins targeting therapeutically relevant NaV subtypes.

Structural and functional insights into the inhibition of human voltage-gated sodium channels by mu-conotoxin KIIIA disulfide isomers.,Tran HNT, McMahon KL, Deuis JR, Vetter I, Schroeder CI J Biol Chem. 2022 Mar;298(3):101728. doi: 10.1016/j.jbc.2022.101728. Epub 2022, Feb 12. PMID:35167877<ref>PMID:35167877</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Schroeder CI]]
[[Category: Tran HNT]]