View source for 7sfa


==Branchiostoma floridae fluorescent protein LanFP10A2==
<StructureSection load='7sfa' size='340' side='right'caption='[[7sfa]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7sfa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_floridae Branchiostoma floridae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JBY:(2S)-2-{(4Z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}propanoic+acid'>JBY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfa OCA], [https://pdbe.org/7sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfa RCSB], [https://www.ebi.ac.uk/pdbsum/7sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C3YRA2_BRAFL C3YRA2_BRAFL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is the first example of a GFP-like fluorescent protein with a stable fluorescent chromophore lacking an imidazolinone ring; instead, it consists of oxidized tyrosine 68 flanked by glycine 67 and alanine 69. bfVFP contains the simplest chromophore reported in fluorescent proteins and was generated from the yellow protein lanFP10A2 by two synergetic mutations, S148H and C166I. The chromophore structure was confirmed crystallographically and by high-resolution mass spectrometry. The photophysical characteristics of bfVFP (323/430 nm, quantum yield 0.33, and Ec 14,300 M(-1) cm(-1) ) make it potentially useful for multicolor experiments to expand the excitation range of available FP biomarkers and Forster resonance energy transfer with blue and cyan fluorescent protein acceptors.

A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.,Roldan-Salgado A, Muslinkina L, Pletnev S, Pletneva N, Pletnev V, Gaytan P Protein Sci. 2022 Mar;31(3):688-700. doi: 10.1002/pro.4265. Epub 2022 Jan 3. PMID:34936154<ref>PMID:34936154</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7sfa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Branchiostoma floridae]]
[[Category: Large Structures]]
[[Category: Muslinkina L]]
[[Category: Pletnev S]]
[[Category: Pletnev VZ]]
[[Category: Pletneva N]]