View source for 7sp5


==Crystal Structure of a Eukaryotic Phosphate Transporter==
<StructureSection load='7sp5' size='340' side='right'caption='[[7sp5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7sp5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serendipita_indica Serendipita indica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4j05 4j05]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SP5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sp5 OCA], [https://pdbe.org/7sp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sp5 RCSB], [https://www.ebi.ac.uk/pdbsum/7sp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sp5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G4TS85_SERID G4TS85_SERID] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phosphate is crucial for structural and metabolic needs, including nucleotide and lipid synthesis, signalling and chemical energy storage. Proton-coupled transporters of the major facilitator superfamily (MFS) are essential for phosphate uptake in plants and fungi, and also have a function in sensing external phosphate levels as transceptors. Here we report the 2.9 A structure of a fungal (Piriformospora indica) high-affinity phosphate transporter, PiPT, in an inward-facing occluded state, with bound phosphate visible in the membrane-buried binding site. The structure indicates both proton and phosphate exit pathways and suggests a modified asymmetrical 'rocker-switch' mechanism of phosphate transport. PiPT is related to several human transporter families, most notably the organic cation and anion transporters of the solute carrier family (SLC22), which are implicated in cancer-drug resistance. We modelled representative cation and anion SLC22 transporters based on the PiPT structure to surmise the structural basis for substrate binding and charge selectivity in this important family. The PiPT structure demonstrates and expands on principles of substrate transport by the MFS transporters and illuminates principles of phosphate uptake in particular.

Crystal structure of a eukaryotic phosphate transporter.,Pedersen BP, Kumar H, Waight AB, Risenmay AJ, Roe-Zurz Z, Chau BH, Schlessinger A, Bonomi M, Harries W, Sali A, Johri AK, Stroud RM Nature. 2013 Mar 31. doi: 10.1038/nature12042. PMID:23542591<ref>PMID:23542591</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7sp5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Serendipita indica]]
[[Category: Bonomi M]]
[[Category: Chau BH]]
[[Category: Finer-Moore J]]
[[Category: Harries W]]
[[Category: Johri AK]]
[[Category: Kumar H]]
[[Category: Pedersen BP]]
[[Category: Risenmay AJ]]
[[Category: Roe-Zurz Z]]
[[Category: Sali A]]
[[Category: Schlessinger A]]
[[Category: Stroud RM]]
[[Category: Waight AB]]