View source for 7ta2


==Crystal structure of the human sperm-expressed surface protein SPACA6==
<StructureSection load='7ta2' size='340' side='right'caption='[[7ta2]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ta2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TA2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ta2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ta2 OCA], [https://pdbe.org/7ta2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ta2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ta2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ta2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SACA6_HUMAN SACA6_HUMAN] Sperm protein required for fusion of sperm with the egg membrane during fertilization (By similarity). May regulate the expression of sperm surface protein DCST2 (By similarity).[UniProtKB:A0A8M9PDM1][UniProtKB:E9Q8Q8]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
SPACA6 is a sperm-expressed surface protein that is critical for gamete fusion during mammalian sexual reproduction. Despite this fundamental role, little is known about how SPACA6 specifically functions. We elucidated the crystal structure of the SPACA6 ectodomain at 2.2-A resolution, revealing a two-domain protein containing a four-helix bundle and Ig-like beta-sandwich connected via a quasi-flexible linker. This structure is reminiscent of IZUMO1, another gamete fusion-associated protein, making SPACA6 and IZUMO1 founding members of a superfamily of fertilization-associated proteins, herein dubbed the IST superfamily. The IST superfamily is defined structurally by its distorted four-helix bundle and a pair of disulfide-bonded CXXC motifs. A structure-based search of the AlphaFold human proteome identified more protein members to this superfamily; remarkably, many of these proteins are linked to gamete fusion. The SPACA6 structure and its connection to other IST-superfamily members provide a missing link in our knowledge of mammalian gamete fusion.

SPACA6 ectodomain structure reveals a conserved superfamily of gamete fusion-associated proteins.,Vance TDR, Yip P, Jimenez E, Li S, Gawol D, Byrnes J, Uson I, Ziyyat A, Lee JE Commun Biol. 2022 Sep 17;5(1):984. doi: 10.1038/s42003-022-03883-y. PMID:36115925<ref>PMID:36115925</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ta2" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Jimenez E]]
[[Category: Lee JE]]
[[Category: Uson I]]
[[Category: Vance TDR]]
[[Category: Yip P]]