View source for 7tgg


==Cryo-EM structure of PilA-N and PilA-C from Geobacter sulfurreducens==
<StructureSection load='7tgg' size='340' side='right'caption='[[7tgg]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7tgg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TGG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tgg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tgg OCA], [https://pdbe.org/7tgg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tgg RCSB], [https://www.ebi.ac.uk/pdbsum/7tgg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tgg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q74D23_GEOSL Q74D23_GEOSL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens were first observed two decades ago, with genetic and biochemical data suggesting that conductive fibres were type IV pili. Recently, an extracellular conductive filament of G. sulfurreducens was found to contain polymerized c-type cytochrome OmcS subunits, not pilin subunits. Here we report that G. sulfurreducens also produces a second, thinner appendage comprised of cytochrome OmcE subunits and solve its structure using cryo-electron microscopy at ~4.3 A resolution. Although OmcE and OmcS subunits have no overall sequence or structural similarities, upon polymerization both form filaments that share a conserved haem packing arrangement in which haems are coordinated by histidines in adjacent subunits. Unlike OmcS filaments, OmcE filaments are highly glycosylated. In extracellular fractions from G. sulfurreducens, we detected type IV pili comprising PilA-N and -C chains, along with abundant B-DNA. OmcE is the second cytochrome filament to be characterized using structural and biophysical methods. We propose that there is a broad class of conductive bacterial appendages with conserved haem packing (rather than sequence homology) that enable long-distance electron transport to chemicals or other microbial cells.

Cryo-EM structure of an extracellular Geobacter OmcE cytochrome filament reveals tetrahaem packing.,Wang F, Mustafa K, Suciu V, Joshi K, Chan CH, Choi S, Su Z, Si D, Hochbaum AI, Egelman EH, Bond DR Nat Microbiol. 2022 Aug;7(8):1291-1300. doi: 10.1038/s41564-022-01159-z. Epub , 2022 Jul 7. PMID:35798889<ref>PMID:35798889</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7tgg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacter sulfurreducens]]
[[Category: Large Structures]]
[[Category: Bond DR]]
[[Category: Chan CH]]
[[Category: Egelman EH]]
[[Category: Hochbaum AI]]
[[Category: Joshi K]]
[[Category: Mustafa K]]
[[Category: Wang F]]