View source for 7thr


==Cryo-electron microscopy of Adeno-associated virus serotype 4 at 2.2 A==
<StructureSection load='7thr' size='340' side='right'caption='[[7thr]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7thr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus_-_4 Adeno-associated virus - 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7THR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7THR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7thr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7thr OCA], [https://pdbe.org/7thr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7thr RCSB], [https://www.ebi.ac.uk/pdbsum/7thr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7thr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O41855_9VIRU O41855_9VIRU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Adeno-associated virus (AAV) is the vector of choice for several approved gene-therapy treatments and is the basis for many ongoing clinical trials. Various strains of AAV exist (referred to as serotypes), each with their own transfection characteristics. Here, a high-resolution cryo-electron microscopy structure (2.2 A) of AAV serotype 4 (AAV4) is presented. The receptor responsible for transduction of the AAV4 clade of AAV viruses (including AAV11, AAV12 and AAVrh32.33) is unknown. Other AAVs interact with the same cell receptor, adeno-associated virus receptor (AAVR), in one of two different ways. AAV5-like viruses interact exclusively with the polycystic kidney disease-like 1 (PKD1) domain of AAVR, while most other AAVs interact primarily with the PKD2 domain. A comparison of the present AAV4 structure with prior corresponding structures of AAV5, AAV2 and AAV1 in complex with AAVR provides a foundation for understanding why the AAV4-like clade is unable to interact with either PKD1 or PKD2 of AAVR. The conformation of the AAV4 capsid in variable regions I, III, IV and V on the viral surface appears to be sufficiently different from AAV2 to ablate binding with PKD2. Differences between AAV4 and AAV5 in variable region VII appear to be sufficient to exclude binding with PKD1.

Cryo-EM structure of adeno-associated virus 4 at 2.2 A resolution.,Zane G, Silveria M, Meyer N, White T, Duan R, Zou X, Chapman M Acta Crystallogr D Struct Biol. 2023 Feb 1;79(Pt 2):140-153. doi: , 10.1107/S2059798322012190. Epub 2023 Jan 20. PMID:36762860<ref>PMID:36762860</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7thr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Adeno-associated virus - 4]]
[[Category: Large Structures]]
[[Category: Chapman MS]]
[[Category: Meyer NL]]
[[Category: Silveria MA]]
[[Category: White TA]]
[[Category: Zane GM]]