View source for 7tn8


==Crystal structure of Zea mays Inositol-tetrakisphosphate Kinase 1 mutant (ZmITPK1-H192A) in complex with InsP6==
<StructureSection load='7tn8' size='340' side='right'caption='[[7tn8]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7tn8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TN8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tn8 OCA], [https://pdbe.org/7tn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tn8 RCSB], [https://www.ebi.ac.uk/pdbsum/7tn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tn8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ITPK1_MAIZE ITPK1_MAIZE] Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 and participates in phytic acid biosynthesis in developing seeds (PubMed:12586875, PubMed:32123645). Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not (PubMed:12586875). Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway (PubMed:12586875). Also able to phosphorylate Ins(3,5,6)P3 but not Ins(1,4,5)P3, Ins(2,4,5)P3, Ins(1,3,4,6)P4 nor Ins(1,3,5,6)P4. Has higher specific activity on Ins(3,4,5,6)P4 than Ins(1,3,4)P3 and Ins(3,5,6)P3 (PubMed:12586875). Can also could use Ins(1,2,5,6)P4 as a substrate (PubMed:12586875). Able to add a beta-phosphate to the 3 positions of Ins(1,2,3,4,5)P5 and to add beta-phosphate to InsP6 to yield 5-InsP7, thus exhibiting InsP6 kinase activity (PubMed:35635723). Has also Ins(1,3,4,5,6)P5 phosphatase activity (PubMed:35635723).<ref>PMID:12586875</ref> <ref>PMID:32123645</ref> <ref>PMID:35635723</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Zea mays]]
[[Category: Shears SB]]
[[Category: Wang H]]
[[Category: Zong G]]