View source for 7tog


==Crystal structure of carbohydrate esterase PbeAcXE, apoenzyme==
<StructureSection load='7tog' size='340' side='right'caption='[[7tog]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7tog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prolixibacter_bellariivorans Prolixibacter bellariivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TOG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tog OCA], [https://pdbe.org/7tog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tog RCSB], [https://www.ebi.ac.uk/pdbsum/7tog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tog ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5M4AV20_9BACT A0A5M4AV20_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Acetylated glucuronoxylan is one of the most common types of hemicellulose in nature. The structure is formed by a beta-(1--&gt;4)-linked D-xylopyranosyl (Xylp) backbone that can be substituted with an acetyl group at O-2 and O-3 positions, and alpha-(1--&gt;2)-linked 4-O-methylglucopyranosyluronic acid (MeGlcpA). Acetyl xylan esterases (AcXE) that target mono- or doubly acetylated Xylp are well characterized; however, the previously studied AcXE from Flavobacterium johnsoniae (FjoAcXE) was the first to remove the acetyl group from 2-O-MeGlcpA-3-O-acetyl-substituted Xylp units, yet structural characteristics of these enzymes remain unspecified. Here, six homologs of FjoAcXE were produced and three crystal structures of the enzymes were solved. Two of them are complex structures, one with bound MeGlcpA and another with acetate. All homologs were confirmed to release acetate from 2-O-MeGlcpA-3-O-acetyl-substituted xylan, and the crystal structures point to key structural elements that might serve as defining features of this unclassified carbohydrate esterase family. Enzymes comprised two domains: N-terminal CBM domain and a C-terminal SGNH domain. In FjoAcXE and all studied homologs, the sequence motif around the catalytic serine is Gly-Asn-Ser-Ile (GNSI), which differs from other SGNH hydrolases. Binding by the MeGlcpA-Xylp ligand is directed by positively charged and highly conserved residues at the interface of the CBM and SGNH domains of the enzyme.

Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.,Penttinen L, Kouhi V, Faure R, Skarina T, Stogios P, Master E, Jurak E Molecules. 2022 Apr 20;27(9). pii: molecules27092655. doi:, 10.3390/molecules27092655. PMID:35566004<ref>PMID:35566004</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7tog" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Prolixibacter bellariivorans]]
[[Category: Di Leo R]]
[[Category: Jurak E]]
[[Category: Master E]]
[[Category: Skarina T]]
[[Category: Stogios PJ]]