View source for 7tue


==Crystal structure of Tapasin in complex with HLA-B*44:05 (T73C)==
<StructureSection load='7tue' size='340' side='right'caption='[[7tue]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7tue]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TUE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tue OCA], [https://pdbe.org/7tue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tue RCSB], [https://www.ebi.ac.uk/pdbsum/7tue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tue ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D5H3J5_HUMAN D5H3J5_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Loading of MHC-I molecules with peptide by the catalytic chaperone tapasin in the peptide loading complex plays a critical role in antigen presentation and immune recognition. Mechanistic insight has been hampered by the lack of detailed structural information concerning tapasin-MHC-I. We present here crystal structures of human tapasin complexed with the MHC-I molecule HLA-B*44:05, and with each of two anti-tapasin antibodies. The tapasin-stabilized peptide-receptive state of HLA-B*44:05 is characterized by distortion of the peptide binding groove and destabilization of the beta(2)-microglobulin interaction, leading to release of peptide. Movements of the membrane proximal Ig-like domains of tapasin, HLA-B*44:05, and beta(2)-microglobulin accompany the transition to a peptide-receptive state. Together this ensemble of crystal structures provides insights into a distinct mechanism of tapasin-mediated peptide exchange.

Structural mechanism of tapasin-mediated MHC-I peptide loading in antigen presentation.,Jiang J, Taylor DK, Kim EJ, Boyd LF, Ahmad J, Mage MG, Truong HV, Woodward CH, Sgourakis NG, Cresswell P, Margulies DH, Natarajan K Nat Commun. 2022 Sep 17;13(1):5470. doi: 10.1038/s41467-022-33153-8. PMID:36115831<ref>PMID:36115831</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7tue" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Boyd LF]]
[[Category: Jiang J]]
[[Category: Kim E]]
[[Category: Margulies DH]]
[[Category: Natarajan K]]