View source for 7tyb


==Salicylate Adenylate PchD from Pseudomonas aeruginosa containing salicyl-AMS==
<StructureSection load='7tyb' size='340' side='right'caption='[[7tyb]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7tyb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TYB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KT0:5-O-[(2-hydroxybenzoyl)sulfamoyl]adenosine'>KT0</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tyb OCA], [https://pdbe.org/7tyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tyb RCSB], [https://www.ebi.ac.uk/pdbsum/7tyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tyb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PCHD_PSEAE PCHD_PSEAE] Involved in the biosynthesis of the siderophore pyochelin (PubMed:8982005). Specifically adenylates salicylate and loads it onto the holo form of PchE via a thioester linkage to the phosphopanthetheine moiety (By similarity). Is also involved in the synthesis of the antifungal antibiotic dihydroaeruginoic acid (Dha or hydroxyphenyl-thiazolinyl-carboxylate), a precursor of pyochelin (PubMed:8982005).[UniProtKB:A0A0H2ZF83]<ref>PMID:8982005</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pseudomonas aeruginosa is an increasingly antibiotic-resistant pathogen that causes severe lung infections, burn wound infections, and diabetic foot infections. P. aeruginosa produces the siderophore pyochelin through the use of a non-ribosomal peptide synthetase (NRPS) biosynthetic pathway. Targeting members of siderophore NRPS proteins is one avenue currently under investigation for the development of new antibiotics against antibiotic-resistant organisms. Here, the crystal structure of the pyochelin adenylation domain PchD is reported. The structure was solved to 2.11 A when co-crystallized with the adenylation inhibitor 5'-O-(N-salicylsulfamoyl)adenosine (salicyl-AMS) and to 1.69 A with a modified version of salicyl-AMS designed to target an active site cysteine (4-cyano-salicyl-AMS). In the structures, PchD adopts the adenylation conformation, similar to that reported for AB3403 from Acinetobacter baumannii.

Rational inhibitor design for Pseudomonas aeruginosa salicylate adenylation enzyme PchD.,Shelton CL, Meneely KM, Ronnebaum TA, Chilton AS, Riley AP, Prisinzano TE, Lamb AL J Biol Inorg Chem. 2022 May 5. pii: 10.1007/s00775-022-01941-8. doi:, 10.1007/s00775-022-01941-8. PMID:35513576<ref>PMID:35513576</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7tyb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Lamb AL]]
[[Category: Meneely KM]]
[[Category: Shelton CL]]