View source for 7ud0


==Room Temperature Drosophila Cryptochrome==
<StructureSection load='7ud0' size='340' side='right'caption='[[7ud0]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ud0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UD0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ud0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ud0 OCA], [https://pdbe.org/7ud0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ud0 RCSB], [https://www.ebi.ac.uk/pdbsum/7ud0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ud0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRY1_DROME CRY1_DROME] Blue light-dependent regulator that is the input of the circadian feedback loop. Has no photolyase activity for cyclobutane pyrimidine dimers or 6-4 photoproducts. Regulation of expression by light suggests a role in photoreception for locomotor activity rhythms. Functions, together with per, as a transcriptional repressor required for the oscillation of peripheral circadian clocks and for the correct specification of clock cells. Genes directly activated by the transcription factors Clock (Clk) and cycle (cyc) are repressed by cry. Necessary for light-dependent magnetosensitivity, an intact circadian system is not required for the magnetoreception mechanism to operate. Required for both the naive and trained responses to magnetic field, consistent with the notion that cry is in the input pathway of magnetic sensing.<ref>PMID:9845369</ref> <ref>PMID:10063806</ref> <ref>PMID:9845370</ref> <ref>PMID:10233998</ref> <ref>PMID:10417378</ref> <ref>PMID:16527739</ref> <ref>PMID:17298948</ref> <ref>PMID:18641630</ref> <ref>PMID:18597555</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fixed-target serial crystallography allows the high-throughput collection of diffraction data from small crystals at room temperature. This methodology is particularly useful for difficult samples that have sensitivity to radiation damage or intolerance to cryoprotection measures; fixed-target methods also have the added benefit of low sample consumption. Here, this method is applied to the structure determination of the circadian photoreceptor cryptochrome (CRY), previous structures of which have been determined at cryogenic temperature. In determining the structure, several data-filtering strategies were tested for combining observations from the hundreds of crystals that contributed to the final data set. Removing data sets based on the average correlation coefficient among equivalent reflection intensities between a given data set and all others was most effective at improving the data quality and maintaining overall completeness. CRYs are light sensors that undergo conformational photoactivation. Comparisons between the cryogenic and room-temperature CRY structures reveal regions of enhanced mobility at room temperature in loops that have functional importance within the CRY family of proteins. The B factors of the room-temperature structure correlate well with those predicted from molecular-dynamics simulations.

Room-temperature serial synchrotron crystallography of Drosophila cryptochrome.,Schneps CM, Ganguly A, Crane BR Acta Crystallogr D Struct Biol. 2022 Aug 1;78(Pt 8):975-985. doi: , 10.1107/S2059798322007008. Epub 2022 Jul 27. PMID:35916222<ref>PMID:35916222</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7ud0" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cryptochrome 3D structures|Cryptochrome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Crane BR]]
[[Category: Schneps CM]]