View source for 7un1


==8-nm repeat of the human sperm tip singlet microtubule==
<StructureSection load='7un1' size='340' side='right'caption='[[7un1]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7un1]] is a 109 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UN1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7un1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7un1 OCA], [https://pdbe.org/7un1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7un1 RCSB], [https://www.ebi.ac.uk/pdbsum/7un1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7un1 ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/TBA1A_HUMAN TBA1A_HUMAN] Lissencephaly due to TUBA1A mutation. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/TBA1A_HUMAN TBA1A_HUMAN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cilium-centrosome complex contains triplet, doublet, and singlet microtubules. The lumenal surfaces of each microtubule within this diverse array are decorated by microtubule inner proteins (MIPs). Here, we used single-particle cryo-electron microscopy methods to build atomic models of two types of human ciliary microtubule: the doublet microtubules of multiciliated respiratory cells and the distal singlet microtubules of monoflagellated human spermatozoa. We discover that SPACA9 is a polyspecific MIP capable of binding both microtubule types. SPACA9 forms intralumenal striations in the B tubule of respiratory doublet microtubules and noncontinuous spirals in sperm singlet microtubules. By acquiring new and reanalyzing previous cryo-electron tomography data, we show that SPACA9-like intralumenal striations are common features of different microtubule types in animal cilia. Our structures provide detailed references to help rationalize ciliopathy-causing mutations and position cryo-EM as a tool for the analysis of samples obtained directly from ciliopathy patients.

SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.,Gui M, Croft JT, Zabeo D, Acharya V, Kollman JM, Burgoyne T, Hoog JL, Brown A Proc Natl Acad Sci U S A. 2022 Oct 11;119(41):e2207605119. doi: , 10.1073/pnas.2207605119. Epub 2022 Oct 3. PMID:36191189<ref>PMID:36191189</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7un1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Acharya V]]
[[Category: Brown A]]
[[Category: Burgoyne T]]
[[Category: Croft JT]]
[[Category: Gui M]]
[[Category: Hoog JL]]
[[Category: Kollman JM]]
[[Category: Zabeo D]]