View source for 7uu5


==Crystal structure of APOBEC3G complex with 5'-Overhang dsRNA==
<StructureSection load='7uu5' size='340' side='right'caption='[[7uu5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7uu5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UU5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uu5 OCA], [https://pdbe.org/7uu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uu5 RCSB], [https://www.ebi.ac.uk/pdbsum/7uu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uu5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/M1GSK9_MACMU M1GSK9_MACMU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
An essential step in restricting HIV infectivity by the antiviral factor APOBEC3G is its incorporation into progeny virions via binding to HIV RNA. However, the mechanism of APOBEC3G capturing viral RNA is unknown. Here, we report crystal structures of a primate APOBEC3G bound to different types of RNAs, revealing that APOBEC3G specifically recognizes unpaired 5'-AA-3' dinucleotides, and to a lesser extent, 5'-GA-3' dinucleotides. APOBEC3G binds to the common 3'A in the AA/GA motifs using an aromatic/hydrophobic pocket in the non-catalytic domain. It binds to the 5'A or 5'G in the AA/GA motifs using an aromatic/hydrophobic groove conformed between the non-catalytic and catalytic domains. APOBEC3G RNA binding property is distinct from that of the HIV nucleocapsid protein recognizing unpaired guanosines. Our findings suggest that the sequence-specific RNA recognition is critical for APOBEC3G virion packaging and restricting HIV infectivity.

Structural basis of sequence-specific RNA recognition by the antiviral factor APOBEC3G.,Yang H, Kim K, Li S, Pacheco J, Chen XS Nat Commun. 2022 Dec 5;13(1):7498. doi: 10.1038/s41467-022-35201-9. PMID:36470880<ref>PMID:36470880</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7uu5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Macaca mulatta]]
[[Category: Synthetic construct]]
[[Category: Chen XJ]]
[[Category: Li S-X]]
[[Category: Yang HJ]]