View source for 7uzx


==Staphylococcus epidermidis RP62a CRISPR effector subcomplex with non-self target RNA bound==
<StructureSection load='7uzx' size='340' side='right'caption='[[7uzx]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7uzx]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis_RP62A Staphylococcus epidermidis RP62A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UZX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.49&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uzx OCA], [https://pdbe.org/7uzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uzx RCSB], [https://www.ebi.ac.uk/pdbsum/7uzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uzx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5HK91_STAEQ Q5HK91_STAEQ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids, they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here, we present seven single-particle cryo-EM structures of the type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP-bound, cognate target RNA-bound, and non-cognate target RNA-bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the type III-A effector complex in a post-RNA cleavage state. The ATP-bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production.

Structures of an active type III-A CRISPR effector complex.,Smith EM, Ferrell S, Tokars VL, Mondragon A Structure. 2022 Aug 4;30(8):1109-1128.e6. doi: 10.1016/j.str.2022.05.013. Epub , 2022 Jun 16. PMID:35714601<ref>PMID:35714601</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7uzx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus epidermidis RP62A]]
[[Category: Ferrell SH]]
[[Category: Mondragon A]]
[[Category: Smith EM]]
[[Category: Tokars VL]]