View source for 7v0b


==Crystal structure of halogenase CtcP from Kitasatospora aureofaciens in complex with FAD==
<StructureSection load='7v0b' size='340' side='right'caption='[[7v0b]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7v0b]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V0B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V0B FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v0b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v0b OCA], [https://pdbe.org/7v0b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v0b RCSB], [https://www.ebi.ac.uk/pdbsum/7v0b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v0b ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CTCP_KITAU CTCP_KITAU] Involved in the biosynthesis of chlorotetracycline (CTC), an important member from antibiotics tetracycline (TC) family, which inhibits protein synthesis in bacteria and is widely involved in clinical therapy, animal feeds and aquaculture. Utilizes FADH(2) supplied by the flavin reductase CtcQ, to catalyze the chlorination of tetracycline (TC) at C7 position, leading to the production of 7-chlorotetracycline. The enzyme forms a lysine chloramine intermediate on an internal lysine residue before transferring the chlorine to the substrate. It is stereo-selective for the 4S (natural) isomer of tetracycline.<ref>PMID:23800859</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Kitasatospora aureofaciens]]
[[Category: Large Structures]]
[[Category: Hou C]]
[[Category: Tsodikov OV]]