View source for 7vcj


==Arginine kinase H227A from Daphnia magna==
<StructureSection load='7vcj' size='340' side='right'caption='[[7vcj]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vcj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Daphnia_magna Daphnia magna]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VCJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vcj OCA], [https://pdbe.org/7vcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vcj RCSB], [https://www.ebi.ac.uk/pdbsum/7vcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vcj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0A7CK57_9CRUS A0A0A7CK57_9CRUS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Arginine kinase (AK) plays a crucial role in the survival of Daphnia magna, a water flea and a common planktonic invertebrate sensitive to water pollution, owing to the production of bioenergy. AK from D. magna (DmAK) has four highly conserved histidine residues, namely, H90, H227, H284, and H315 in the amino acid sequence. In contrast to DmAK WT (wild type), the enzyme activity of the H227A mutant decreases by 18%. To identify the structure-function relationship of this H227A mutant enzyme, the crystal 3D X-ray structure has been determined and an unfolding assay using anilino-1-naphthalenesulfonic acid (ANS) fluorescence has been undertaken. The results revealed that when compared to the DmAK WT, the hydrogen bonding between H227 and A135 was broken in the H227A crystal structure. This suggests that H227 residue, closed to the arginine binding site, plays an important role in maintaining the structural stability and maximizing the enzyme activity through hydrogen bonding with the backbone oxygen of A135.

Crystal Structure of H227A Mutant of Arginine Kinase in Daphnia magna Suggests the Importance of Its Stability.,Kim DS, Jang K, Kim WS, Ryu M, Park JH, Kim YJ Molecules. 2022 Jan 28;27(3). pii: molecules27030884. doi:, 10.3390/molecules27030884. PMID:35164149<ref>PMID:35164149</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vcj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Arginine kinase 3D structures|Arginine kinase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Daphnia magna]]
[[Category: Large Structures]]
[[Category: Jang K]]
[[Category: Kim DS]]
[[Category: Kim WS]]
[[Category: Kim YJ]]
[[Category: Park JH]]