View source for 7vda


==2.26 A structure of the glutamate dehydrogenase==
<StructureSection load='7vda' size='340' side='right'caption='[[7vda]], [[Resolution|resolution]] 2.26&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VDA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VDA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.26&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vda FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vda OCA], [https://pdbe.org/7vda PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vda RCSB], [https://www.ebi.ac.uk/pdbsum/7vda PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vda ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution structures of biomacromolecules in solution. However, air-water interface induced preferred orientations, dissociation or denaturation of biomacromolecules during cryo-vitrification remains a limiting factor for many specimens. To solve this bottleneck, we developed a cryo-EM support film using 2D crystals of hydrophobin HFBI. The hydrophilic side of the HFBI film adsorbs protein particles via electrostatic interactions and sequesters them from the air-water interface, allowing the formation of sufficiently thin ice for high-quality data collection. The particle orientation distribution can be regulated by adjusting the buffer pH. Using this support, we determined the cryo-EM structures of catalase (2.29 A) and influenza haemagglutinin trimer (2.56 A), which exhibited strong preferred orientations using a conventional cryo-vitrification protocol. We further show that the HFBI film is suitable to obtain high-resolution structures of small proteins, including aldolase (150 kDa, 3.28 A) and haemoglobin (64 kDa, 3.6 A). Our work suggests that HFBI films may have broad future applications in increasing the success rate and efficiency of cryo-EM.

A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI.,Fan H, Wang B, Zhang Y, Zhu Y, Song B, Xu H, Zhai Y, Qiao M, Sun F Nat Commun. 2021 Dec 14;12(1):7257. doi: 10.1038/s41467-021-27596-8. PMID:34907237<ref>PMID:34907237</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7vda" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Fan HC]]
[[Category: Sun F]]
[[Category: Zhang Y]]