View source for 7vgr


==SARS-CoV-2 M protein dimer (long form) in complex with YN7756_1 Fab==
<StructureSection load='7vgr' size='340' side='right'caption='[[7vgr]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vgr]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgr OCA], [https://pdbe.org/7vgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgr RCSB], [https://www.ebi.ac.uk/pdbsum/7vgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VME1_SARS2 VME1_SARS2] Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed:33229438). May act by binding cytoplasmic c-terminus of S (PubMed:33229438).[HAMAP-Rule:MF_04202][PROSITE-ProRule:PRU01275]<ref>PMID:33229438</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The coronavirus membrane protein (M) is the most abundant viral structural protein and plays a central role in virus assembly and morphogenesis. However, the process of M protein-driven virus assembly are largely unknown. Here, we report the cryo-electron microscopy structure of the SARS-CoV-2 M protein in two different conformations. M protein forms a mushroom-shaped dimer, composed of two transmembrane domain-swapped three-helix bundles and two intravirion domains. M protein further assembles into higher-order oligomers. A highly conserved hinge region is key for conformational changes. The M protein dimer is unexpectedly similar to SARS-CoV-2 ORF3a, a viral ion channel. Moreover, the interaction analyses of M protein with nucleocapsid protein (N) and RNA suggest that the M protein mediates the concerted recruitment of these components through the positively charged intravirion domain. Our data shed light on the M protein-driven virus assembly mechanism and provide a structural basis for therapeutic intervention targeting M protein.

Structure of SARS-CoV-2 membrane protein essential for virus assembly.,Zhang Z, Nomura N, Muramoto Y, Ekimoto T, Uemura T, Liu K, Yui M, Kono N, Aoki J, Ikeguchi M, Noda T, Iwata S, Ohto U, Shimizu T Nat Commun. 2022 Aug 5;13(1):4399. doi: 10.1038/s41467-022-32019-3. PMID:35931673<ref>PMID:35931673</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vgr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Severe acute respiratory syndrome coronavirus 2]]
[[Category: Ohto U]]
[[Category: Shimizu T]]
[[Category: Zhang Z]]