View source for 7vjk


==class II photolyase MmCPDII semiquinone to fully reduced TR-SFX studies (100 us time-point)==
<StructureSection load='7vjk' size='340' side='right'caption='[[7vjk]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vjk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei_Go1 Methanosarcina mazei Go1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjk OCA], [https://pdbe.org/7vjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjk RCSB], [https://www.ebi.ac.uk/pdbsum/7vjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q8PYK9_METMA Q8PYK9_METMA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flavin coenzymes are universally found in biological redox reactions. DNA photolyases, with their flavin chromophore (FAD), utilize blue light for DNA repair and photoreduction. The latter process involves two single-electron transfers to FAD with an intermittent protonation step to prime the enzyme active for DNA repair. Here we use time-resolved serial femtosecond X-ray crystallography to describe how light-driven electron transfers trigger subsequent nanosecond-to-microsecond entanglement between FAD and its Asn/Arg-Asp redox sensor triad. We found that this key feature within the photolyase-cryptochrome family regulates FAD re-hybridization and protonation. After first electron transfer, the FAD(*-) isoalloxazine ring twists strongly when the arginine closes in to stabilize the negative charge. Subsequent breakage of the arginine-aspartate salt bridge allows proton transfer from arginine to FAD(*-). Our molecular videos demonstrate how the protein environment of redox cofactors organizes multiple electron/proton transfer events in an ordered fashion, which could be applicable to other redox systems such as photosynthesis.

Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme.,Maestre-Reyna M, Yang CH, Nango E, Huang WC, Ngurah Putu EPG, Wu WJ, Wang PH, Franz-Badur S, Saft M, Emmerich HJ, Wu HY, Lee CC, Huang KF, Chang YK, Liao JH, Weng JH, Gad W, Chang CW, Pang AH, Sugahara M, Owada S, Hosokawa Y, Joti Y, Yamashita A, Tanaka R, Tanaka T, Luo F, Tono K, Hsu KC, Kiontke S, Schapiro I, Spadaccini R, Royant A, Yamamoto J, Iwata S, Essen LO, Bessho Y, Tsai MD Nat Chem. 2022 Apr 7. pii: 10.1038/s41557-022-00922-3. doi:, 10.1038/s41557-022-00922-3. PMID:35393554<ref>PMID:35393554</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vjk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanosarcina mazei Go1]]
[[Category: Bessho Y]]
[[Category: Chang C-W]]
[[Category: Chang Y-K]]
[[Category: Emmerich H-J]]
[[Category: Essen L-O]]
[[Category: Franz-Badur S]]
[[Category: Gad W]]
[[Category: Hosokawa Y]]
[[Category: Huang K-F]]
[[Category: Huang W-C]]
[[Category: Iwata S]]
[[Category: Joti Y]]
[[Category: Kiontke S]]
[[Category: Lee C-C]]
[[Category: Liao J-H]]
[[Category: Luo FJ]]
[[Category: Maestre-Reyna M]]
[[Category: Nango E]]
[[Category: Ngura Putu EPG]]
[[Category: Owada S]]
[[Category: Pang AH]]
[[Category: Royant A]]
[[Category: Saft M]]
[[Category: Sugahara M]]
[[Category: Tanaka R]]
[[Category: Tanaka T]]
[[Category: Tono K]]
[[Category: Tsai M-D]]
[[Category: Wang P-H]]
[[Category: Weng J-H]]
[[Category: Wu H-Y]]
[[Category: Wu W-J]]
[[Category: Yamamoto J]]
[[Category: Yamashita A]]
[[Category: Yang C-H]]