View source for 7vm9


==Solution NMR structures of DNA minidumbbell formed with two regular CTTTG pentaloops==
<StructureSection load='7vm9' size='340' side='right'caption='[[7vm9]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VM9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vm9 OCA], [https://pdbe.org/7vm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vm9 RCSB], [https://www.ebi.ac.uk/pdbsum/7vm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vm9 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Minidumbbell (MDB) is a noncanonical DNA structure found to form in several pyrimidine-rich short tandem repeats associated with neurodegenerative diseases. The most recently reported MDB contains two pentaloops formed by ATTCT repeats. Here, we studied the effects of a purine residue and a backbone discontinuous site on the structure and thermal stability of MDBs containing two pentaloops. It was found that a purine as the fourth loop residue improved the thermal stability of MDBs containing two regular pentaloops, while a backbone discontinuous site between the third and fourth, or between the fourth and fifth loop residues enhanced the thermal stability of MDBs containing a regular and a quasi pentaloops. The results of this study provide new insights into the sequence criteria and structural basis of MDBs.

A purine and a backbone discontinuous site alter the structure and thermal stability of DNA minidumbbells containing two pentaloops.,Ngai CK, Lam SL, Lee HK, Guo P FEBS Lett. 2022 Jan 21. doi: 10.1002/1873-3468.14293. PMID:35060128<ref>PMID:35060128</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7vm9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Guo P]]
[[Category: Ngai CK]]