View source for 7vqh


==Solution NMR structure of Escherichia coli Total Lipid Extract Bicelle bound VR18 Antimicrobial Peptide==
<StructureSection load='7vqh' size='340' side='right'caption='[[7vqh]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VQH FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vqh OCA], [https://pdbe.org/7vqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vqh RCSB], [https://www.ebi.ac.uk/pdbsum/7vqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vqh ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Contact lens wearers are at an increased risk of developing Pseudomonas-associated corneal keratitis, which can lead to a host of serious ocular complications. Despite the use of topical antibiotics, ocular infections remain a major clinical problem, and a strategy to avoid Pseudomonas-associated microbial keratitis is urgently required. The hybrid peptide VR18 (VARGWGRKCPLFGKNKSR) was designed to have enhanced antimicrobial properties in the fight against Pseudomonas-induced microbial keratitis, including contact lens-related keratitis. In this paper, VR18's modes of action against Pseudomonas membranes were shown by live cell Raman spectroscopy, live cell NMR, live-cell fluorescence microscopy and measures taken using sparsely tethered bilayer lipid membrane bacterial models to be via a bacterial-specific membrane disruption mechanism. The high affinity and selectivity of the peptide were then demonstrated using in vivo, in vitro and ex vivo models of Pseudomonas infection. The extensive data presented in this work suggests that topical employment of the VR18 peptide would be a potent therapeutic agent for the prevention or remedy of Pseudomonas-associated microbial keratitis.

A rationally designed synthetic antimicrobial peptide against Pseudomonas-associated corneal keratitis: Structure-function correlation.,Mohid SA, Sharma P, Alghalayini A, Saini T, Datta D, Willcox MDP, Ali H, Raha S, Singha A, Lee D, Sahoo N, Cranfield CG, Roy S, Bhunia A Biophys Chem. 2022 Jul;286:106802. doi: 10.1016/j.bpc.2022.106802. Epub 2022 Mar , 22. PMID:35605494<ref>PMID:35605494</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Bhunia A]]
[[Category: Mohid SA]]