View source for 7vtn


==Cryo-EM structure of the Cas13bt3-crRNA-target RNA ternary complex==
<StructureSection load='7vtn' size='340' side='right'caption='[[7vtn]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7vtn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Planctomycetes_bacterium Planctomycetes bacterium] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VTN FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vtn OCA], [https://pdbe.org/7vtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vtn RCSB], [https://www.ebi.ac.uk/pdbsum/7vtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vtn ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/A0A660UUL5_9BACT A0A660UUL5_9BACT]] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type VI CRISPR-Cas13 effector enzymes catalyze RNA-guided RNA cleavage and have been harnessed for various technologies, such as RNA detection, targeting, and editing. Recent studies identified Cas13bt3 (also known as Cas13X.1) as a miniature Cas13 enzyme, which can be used for knockdown and editing of target transcripts in mammalian cells. However, the action mechanism of the compact Cas13bt3 remains unknown. Here, we report the structures of the Cas13bt3-guide RNA complex and the Cas13bt3-guide RNA-target RNA complex. The structures revealed how Cas13bt3 recognizes the guide RNA and its target RNA and provided insights into the activation mechanism of Cas13bt3, which is distinct from those of the other Cas13a/d enzymes. Furthermore, we rationally engineered enhanced Cas13bt3 variants and ultracompact RNA base editors. Overall, this study improves our mechanistic understanding of the CRISPR-Cas13 enzymes and paves the way for the development of efficient Cas13-mediated transcriptome modulation technologies.

Structure and engineering of the minimal type VI CRISPR-Cas13bt3.,Nakagawa R, Kannan S, Altae-Tran H, Takeda SN, Tomita A, Hirano H, Kusakizako T, Nishizawa T, Yamashita K, Zhang F, Nishimasu H, Nureki O Mol Cell. 2022 Sep 1;82(17):3178-3192.e5. doi: 10.1016/j.molcel.2022.08.001. Epub, 2022 Aug 25. PMID:36027912<ref>PMID:36027912</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7vtn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Planctomycetes bacterium]]
[[Category: Synthetic construct]]
[[Category: Feng Z]]
[[Category: Han A]]
[[Category: Hirano H]]
[[Category: Kusakizako T]]
[[Category: Nakagawa R]]
[[Category: Nishimasu H]]
[[Category: Nureki O]]
[[Category: Soumya K]]
[[Category: Takeda NS]]
[[Category: Tomita A]]
[[Category: Tomohiro N]]
[[Category: Yamashita K]]