View source for 7way


==PlmCasX-sgRNAv1-dsDNA ternary complex at nts loading state==
<StructureSection load='7way' size='340' side='right'caption='[[7way]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7way]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Planctomycetes_bacterium Planctomycetes bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7way FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7way OCA], [https://pdbe.org/7way PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7way RCSB], [https://www.ebi.ac.uk/pdbsum/7way PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7way ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
A compact protein with a size of &lt;1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is less robust than that of the widely used S. pyogenes Cas9. Here, we show that structural features of two CasX homologs and their guide RNAs affect the R-loop complex assembly and DNA cleavage activity. Cryo-EM-based structural engineering of either the CasX protein or the guide RNA produced two new CasX genome editors (DpbCasX-R3-v2 and PlmCasX-R1-v2) with significantly improved DNA manipulation efficacy. These results advance both the mechanistic understanding of CasX and its application as a genome-editing tool.

Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.,Tsuchida CA, Zhang S, Doost MS, Zhao Y, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brotzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JG Mol Cell. 2022 Mar 17;82(6):1199-1209.e6. doi: 10.1016/j.molcel.2022.02.002. Epub , 2022 Feb 25. PMID:35219382<ref>PMID:35219382</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 7way" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Planctomycetes bacterium]]
[[Category: Liu JJG]]
[[Category: Zhang S]]