View source for 7whw


==Cryo-EM structure of Dnf1 from Saccharomyces cerevisiae in detergent with AMPPCP (E1-ATP state)==
<StructureSection load='7whw' size='340' side='right'caption='[[7whw]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7whw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WHW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7whw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7whw OCA], [https://pdbe.org/7whw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7whw RCSB], [https://www.ebi.ac.uk/pdbsum/7whw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7whw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATC5_YEAST ATC5_YEAST] Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of glucosylceramide, phosphatidylcholine, phosphatidylethanolamine, and small amounts of phosphatidylserine from the lumenal to the cytosolic leaflet of the cell membrane and ensures the maintenance of asymmetric distribution of phospholipids (PubMed:12631737, PubMed:22308393, PubMed:22791719, PubMed:23302692, PubMed:31786280, PubMed:33060204, PubMed:33320091, PubMed:35294892). Does not appear to transport sphingomyelin, inositol phosphoceramide, or phosphatidic acid (PubMed:12631737, PubMed:22308393, PubMed:33320091). Required for efficient endocytosis (PubMed:12631737).<ref>PMID:12631737</ref> <ref>PMID:22308393</ref> <ref>PMID:22791719</ref> <ref>PMID:23302692</ref> <ref>PMID:31786280</ref> <ref>PMID:33060204</ref> <ref>PMID:33320091</ref> <ref>PMID:35294892</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type 4 P-type ATPases (P4-ATPases) actively and selectively translocate phospholipids across membrane bilayers. Driven by ATP hydrolysis, P4-ATPases undergo conformational changes during lipid flipping. It is unclear how the active flipping states of P4-ATPases are regulated in the lipid membranes, especially for phosphatidylcholine (PC)-flipping P4-ATPases whose substrate, PC, is a substantial component of membranes. Here, we report the cryoelectron microscopy structures of a yeast PC-flipping P4-ATPase, Dnf1, in lipid environments. In native yeast lipids, Dnf1 adopts a conformation in which the lipid flipping pathway is disrupted. Only when the lipid composition is changed can Dnf1 be captured in the active conformations that enable lipid flipping. These results suggest that, in the native membrane, Dnf1 may stay in an idle conformation that is unable to support the trans-membrane movement of lipids. Dnf1 may have altered conformational preferences in membranes with different lipid compositions.

Conformational changes of a phosphatidylcholine flippase in lipid membranes.,Xu J, He Y, Wu X, Li L Cell Rep. 2022 Mar 15;38(11):110518. doi: 10.1016/j.celrep.2022.110518. PMID:35294892<ref>PMID:35294892</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7whw" style="background-color:#fffaf0;"></div>

==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: He Y]]
[[Category: Li L]]
[[Category: Wu X]]
[[Category: Xu J]]